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Systemic administration of STING agonist inhibits melanoma growth and stimulates an immunogenic tumor microenvironment. A–C B16-F10-bearing mice were intravenously injected with different concentrations of diABZI or vehicle (n = 5 mice in each group). A Tumor weights, B tumor growth curves and C overall survival curves are shown. Tumor-infiltrating D–G T cells, H, I B cells, J, K monocytes (CD11b+ Ly6Chi), and neutrophils (CD11b+ Ly6G+) were analyzed using flow cytometry at 48 h after last administration. Results displayed are representative of at least two independent experiments and are presented as the mean ± SD (n = 3–5 biologically independent samples). n.s., not significant, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the vehicle group

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Systemic administration of STING agonist promotes myeloid cells maturation and antitumor immunity through regulating hematopoietic stem and progenitor cell fate

doi: 10.1007/s00262-023-03502-7

Figure Lengend Snippet: Systemic administration of STING agonist inhibits melanoma growth and stimulates an immunogenic tumor microenvironment. A–C B16-F10-bearing mice were intravenously injected with different concentrations of diABZI or vehicle (n = 5 mice in each group). A Tumor weights, B tumor growth curves and C overall survival curves are shown. Tumor-infiltrating D–G T cells, H, I B cells, J, K monocytes (CD11b+ Ly6Chi), and neutrophils (CD11b+ Ly6G+) were analyzed using flow cytometry at 48 h after last administration. Results displayed are representative of at least two independent experiments and are presented as the mean ± SD (n = 3–5 biologically independent samples). n.s., not significant, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the vehicle group

Article Snippet: For immunofluorescence staining, the tissue slides were incubated with specific primary antibodies against CD3, CD8, CD4, B220, CD11b, and arginase I (Arg-1) (Servicebio, Wuhan, China), followed by incubation with anti-mouse/rabbit Alexa Fluor secondary antibodies (Cell Signaling Technology).

Techniques: Injection, Flow Cytometry